Moreover, YQJD promoted the secretion of IL-4 to protect the cornea, leading to the mitigation of corneal immunopathological damage. YQJD increased the proportion of CD4 +Foxp3 + Treg cells also, in the cornea, YQJD inhibited the aggregation of macrophages and CD4 + cells and reduced the proportion of Th17 cells and other pro-inflammatory cells. YQJD alleviated corneal inflammation by enhancing the STAT5 signaling pathway, thereby promoting the differentiation of CD4 +CD25 +Foxp3 + Treg cells, increasing the levels of anti-inflammatory cytokines such as IL-10 and TGF-ОІ, and maintaining immune tolerance. Furthermore, the activation status of the STAT5 signaling pathway was examined by protein blotting, and the effect of YQJD on Treg cells through inhibition of the STAT5 pathway was observed in vitro. The levels of the cytokines IL-10 and TGF-ОІ in serum and splenocyte culture supernatants were detected by enzyme-linked immunosorbent assay. The CD4 +Foxp3 + Tregs were assessed by immunofluorescence in the cornea. The abundance of CD4 +CD25 +Foxp3 + regulatory T (Treg) cells was determined by flow cytometry in peripheral blood and spleen cells.

After inducing recurrence by ultraviolet irradiation, the ocular surfaces of different groups of mice were observed using a slit lamp and photographed, and ocular surface scores were calculated. Sixty female BALB/c mice were randomly divided into three groups: a normal control group (Naive), a recurrence model group (Re), and a YQJD group. This study aimed to investigate the effect of Yiqi Jiedu (YQJD) formula on the repair of corneal lesions in mice with recurrent herpes simplex virus keratitis (HSK). 3Shanghai Eye Disease Control Center, Shanghai, China.

2Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

1Department of Ophthalmology, Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Shuyu Xiao 1 вЂ , Yang Yang 2 вЂ , Wanhong Miao 1, Chunming Lyu 2, Jinhua Tao 3* and Ying Yu 1*

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